A rapid DNA isolation procedure from petri dish grown clinical bacterial isolates.

Gardnerella vaginalis, a pleomorphic Gram variable bacteria (1), with a Gram positive cell wall (2). that stains predominantly as Gram negative is associated in bacterial vaginosis (1). We have described a lysozyme-SDS-proteinase K method for the isolation of DNA from 250 ml Casman broth cultures requiring about 60 to 72 hours (3). However, this was not convenient for comparative restriction endonuclease analysis (REA) studies involving a large number of clinical isolates. Here, we describe a procedure that has been modified from broth cultures to petri dish cultures and the time required has been shortened to less